Coding
Part:BBa_K1690000:Design
Designed by: Anna Sosnova, Pavel Zach Group: iGEM15_Czech_Republic (2015-09-16)
Mating pheromone (S. cerevisiae)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In the genome, the secretion tag is followed by a three copies of the coding sequence for the actual peptide. We had to amplify only the secretion tag first, and then added the DNA sequence of the peptide using different reverse primer.
Source
Obtained from the genome of S. cerevisiae [MF(ALPHA)1 locus].