Coding

Part:BBa_K1690000:Design

Designed by: Anna Sosnova, Pavel Zach   Group: iGEM15_Czech_Republic   (2015-09-16)


Mating pheromone (S. cerevisiae)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the genome, the secretion tag is followed by a three copies of the coding sequence for the actual peptide. We had to amplify only the secretion tag first, and then added the DNA sequence of the peptide using different reverse primer.


Source

Obtained from the genome of S. cerevisiae [MF(ALPHA)1 locus].

References